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File:Depth Coded Phalloidin Stained Actin Filaments Cancer Cell.png

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Original file (3,176 × 2,472 pixels, file size: 9.86 MB, MIME type: image/png)

Summary

Description
English: A z-projection of an osteosarcoma cell, stained with phalloidin to visualise actin filaments. The image was taken on a confocal microscope, and the subsequent deconvolution was done using an experimentally derived point spread function.

Image Parameters
Microscope: Zeiss LSM 780 Confocal Microscope
Pinhole [m]: 23 µm
Total Size-Width: 133.69 µm
Total Size-Height: 134.91 µm
Total Size-Depth: 6.48 µm
Voxel-Width: 42.0 nm (pixel size)
Voxel-Height: 42.0 nm (pixel size)
Voxel-Depth: 80.0 nm
Resolution: 23.5938 pixels per micron
Zoom: 1.0
Objective: Plan-Apochromat 63x/1.40 Oil DIC

Numerical aperture: 1.40
Date
Source Own work
Author Howard Vindin

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I, the copyright holder of this work, hereby publish it under the following license:
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inception

30 June 2014

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Date/TimeThumbnailDimensionsUserComment
current20:14, 24 July 2015Thumbnail for version as of 20:14, 24 July 20153,176 × 2,472 (9.86 MB)CmdrjamesonCompressed with pngout. Reduced by 12950kB (56% decrease).
06:58, 20 May 2015Thumbnail for version as of 06:58, 20 May 20153,176 × 2,472 (22.5 MB)Methylated603User created page with UploadWizard

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