Dr. Marwan Jawad Msarah

Screening of new source of novel and industrially useful enzymes is a key research pursuit
in enzyme biotechnology. The study aims to report the characteristics of novel thermophilic
microorganisms isolated from Sungai Klah (SK) Hot Spring, Perak, Malaysia, that can
produce α-amylase.

Bacillus cereus is a ubiquitous foodborne pathogen, can cause food poisoning, leading to infections, have two major types of food poisoning emetic and diarrheal. Foods rich in protein such as meat are associated with foodborne outbreaks of diarrhea caused by B. cereus. The aim of this study is to isolate and identify B. cereus from ready to eat (RTE) meat curry from restaurants in Malaysia and to detect hblD pathogenic gene of B. cereus isolates. Mannitol egg yolk polymyxin agar was used as a… عرض المزيد

Bacillus cereus is a ubiquitous foodborne pathogen, can cause food poisoning, leading to infections, have two major types of food poisoning emetic and diarrheal. Foods rich in protein such as meat are associated with foodborne outbreaks of diarrhea caused by B. cereus. The aim of this study is to isolate and identify B. cereus from ready to eat (RTE) meat curry from restaurants in Malaysia and to detect hblD pathogenic gene of B. cereus isolates. Mannitol egg yolk polymyxin agar was used as a selective isolation medium. Commercially available kits and boiling methods were used for DNA extraction, samples acquired from restaurants were examined for the presence of Hemolysin BL gene by polymerase chain reaction (PCR). Among all isolates, twenty-four of B. cereus isolates detected for HBL enterotoxin production by the discontinuous pattern on HBL sheep blood agar then confirmed by biochemical tests. More than 58.33 % of the isolate showed discontinuous hemolysis pattern on HBl blood agar and 29.16% of the samples were shown positive for hblD gene that can cause diarrhea with the size of 807bp on gel. This study demonstrated that RTE meat curry was a potential source for entero-toxigenic B. cereus and the presence of the hblD toxin genes for the HBL complex in the isolates tested were highly associated. Therefore, these meat curry isolates should be regarded as potential toxin producers. عرض أقل

Thermophilic bacteria are refractory and widely spread, relatively in elevated temperature habitats such as in hot spring. Isolation of novel thermophiles for biotechnological and industrial usage has come into prominence in recent years because of their ability to possess unique enzymes under extreme circumstances having high thermal activity and stability at high temperatures. The aims of this study are the isolation of thermophilic and screening of the extracellular enzymes from thermophilic… عرض المزيد

Thermophilic bacteria are refractory and widely spread, relatively in elevated temperature habitats such as in hot spring. Isolation of novel thermophiles for biotechnological and industrial usage has come into prominence in recent years because of their ability to possess unique enzymes under extreme circumstances having high thermal activity and stability at high temperatures. The aims of this study are the isolation of thermophilic and screening of the extracellular enzymes from thermophilic bacteria using conventional methods (morphological, physiological and biochemical tests), and screening for the production of extracellular enzymes. عرض أقل

Extreme environments have been the interest of numerous studies and scientific publications. For many years, these environmental roles, which are characterised by high or low temperatures, high-pressure, low pH, high salt concentrations and companions of two or more extreme parameters. For many years, these environmental niches have increased in importance due to extreme microorganisms diversity and their potential returns of several industrial production processes concerning the food fields… عرض المزيد

Extreme environments have been the interest of numerous studies and scientific publications. For many years, these environmental roles, which are characterised by high or low temperatures, high-pressure, low pH, high salt concentrations and companions of two or more extreme parameters. For many years, these environmental niches have increased in importance due to extreme microorganisms diversity and their potential returns of several industrial production processes concerning the food fields, medical and pharmaceutical. At present, it's possible to detect life in most extreme environments, from the deep sea hot springs, depth of the oceans, deep inside the earth crust, harsh chemical environments, and high radiation environments through metagenomics analysis. عرض أقل

The aim of this study includes to isolate thermophilic bacteria from hot spring and
screen for important enzyme activities

The purpose of the study was to elucidate the effect of preparation methods hard-boiled half-boiled & raw on the
digestibility of protein and the amino acid composition as well as the protein quality of nutrient enriched Malaysian
eggs. The amino acid content was determined using HPLC

The aim of the study was to isolate and identify Bacillus cereus from meat curry and to subtype
the isolated B. cereus using RAPD-PCR and antibiotic resistance pattern. Ready to eat (RTE)
meat curry samples purchased from 12 different restaurants at Kajang, Serdang and KL Sentral
regions located in Selangor and Kuala Lumpur, Malaysia.