Creating 3D models of a coastal cliff with the iPhone LiDAR sensor

The iPhone LiDAR sensor is used to generate accurate 3D representations of a coastal cliff. The LiDAR sensor allows the rapid generation of detailed 3D models giving insights into object size, volume and geometry. This enables precise measurements of the cliff erosion processes over time. See Luetzenburg et al.p3520.

Mice in motion

Animal behavior is built from basic units of movement, analogous to syllables in human language. In mice, these ‘syllables’ have subsecond duration and include rears, turns, darts and more. Pose trajectories for several example syllables are shown here. To help with visualization, consecutive frames have been shifted horizontally before superimposing them. See Lin et al

Unveiling gut architecture using high-dimensional immunofluorescence

To visualize the niche of murine intestinal mononuclear phagocytes and their association with other immune and stromal cells, a ‘Swiss roll’ section of the distal portion of the small intestine was stained for CODEX-PhenoCycler multiplexed imaging. This method, in conjunction with the tissue-rolling technique, allows the gut architecture to be dissected at single-cell resolution. See Monasterio et al.

Microfluidic system to isolate neuronal compartments

Neurons of the dorsal root ganglia grow along channels of microfluidic chambers. This enables the isolation and subsequent study of membrane protein dynamics in discrete neuronal somas (orange) and axons (blue). See Tyagi et al.

Illuminating the mechanisms of small-molecule-induced protein proximity and degradation

Small-molecule-induced ternary complexes of NanoLuciferase (NLuc)-fused E3 ligase and a HaloTag-fused neosubstrate are shown. The induced proximity between the two proteins leads to bioluminescence resonance energy transfer (BRET) between the blue luminescent NLuc and the pink BRET acceptor. The formation of these ternary complexes leads to the ubiquitination and subsequent degradation of the neosubstrates by the proteasome as shown in the background.

Classification of T cell behavior

Tracking the trajectory of engineered T cells targeted against patient-derived cancer organoids reveals different patterns of behavior. Cancer organoids are displayed in white, and T cells with different behavioral profiles are displayed in different colors. Tracks from live confocal imaging are loosely traced by hand to portray the plasticity of T cell movement. See Alieva et al.

MXene jeobeon fan

A scanning electron microscopy image of a Ti3C2Tx MXene particle partially exfoliated by high shear mixing and imaged at the Korea Advanced Institute of Science and Technology (KAIST)/National NanoFab Center (NNFC) in Daejeon, South Korea, during a visit from Philadelphia, USA. The fan has close to the 18 ribs commonly found on traditional Korean fans, while the surrounding particles resemble falling cherry blossom petals that bloom throughout South Korea and Philadelphia. See Downes et al.

Illuminating digital cells on a microscopic stage.

A detailed digital reconstruction of an animal cell receiving light cast from afar. This represents the transformation of cells from microscopy images into a form suitable for accurate optical modeling of electromagnetic wave propagation.

Tumor cell captured via magnetic beads functionalized with tetrahedral DNA frameworks

Scanning electron micrograph of streptavidin-labeled magnetic beads (green) bound to biotin-labeled tetrahedral DNA frameworks anchored with aptamers to a HepG2 cell (pink). See Chen, Y. et al.

Studying human brain vascular cells with single-cell transcriptomics and organoids

Human brain vascular cells, including endothelial and mural cells, can be purified with FACS and used in many downstream applications, including cell culture, transcriptomics and organoid transplants. This image shows vascular cells labeled with GFP and transplanted on top of induced pluripotent stem cell (iPSC)-derived cortical organoids. See Crouch et al. p603

Terpene cyclization inside the resorcin[4]arene capsule

Hexameric capsules that self-assemble from resorcin[4]arene are shown in this rendered image. Luminous oxygen and hydrogen atoms highlight the hydrogen bond network holding the assemblies together. In the foreground, the capsule is open, revealing its content: a cyclized presilphiperfolanol molecule. See Cornu et al.

Label-free X-ray microscopy of nanomedicines and organelles in intact single cells at nanometer resolution using synchrotron radiation

A composite image of a single cell visualized in 3D and a synchrotron radiation facility. The method uses X-rays generated via synchrotron radiation and enables the subcellular localization of nanomedicines in single cells, at nanometer resolution, as a robust approach to characterize interactions between nanomaterials and cells. See Cao et al.