In this protocol, the tissue clearing process is carried out using a multiwell plate and insert, which allows the clearing steps to be conducted in parallel across multiple samples.

ChromEMT combines a fluorescent DNA binding dye that selectively enhances DNA and nucleosomes in electron microscopy with multi-tilt tomography, to enable the imaging and reconstruction of nuclear chromatin ultrastructure and 3D organization.

We present a protocol detailing the extraction and quantification of glycosaminoglycans extracted from biofluids by using ultra-high-performance liquid chromatography combined with triple-quadrupole mass spectrometry.

This protocol outlines a medium-throughput strategy based on combinatorial dye staining of pools of effector T cells to screen in parallel the reactivity of up to hundreds of T cell receptors against patient primary tissues or panels of antigens.

Standardized neuroimaging protocols are essential to advancing comparability and replicability. Now, a comprehensive magnetic resonance imaging acquisition and analysis protocol from the Rhineland Study provides a valuable framework for image acquisition and analysis that is suitable for both large- and small-scale neuroimaging studies.

A neuroimaging protocol presenting versatile high-resolution and time-efficient MRI acquisition and processing strategies suitable for large-scale and long-term population studies, which include structural, diffusion-weighted and functional MRI.

The members of Independent SAGE reflect on their experience in setting up, developing and running a science communication platform and service to the public during the COVID-19 pandemic

This deep learning platform enables large chemical spaces to be mined to identify substructures underlying predicted activity, with explainable reasoning behind the predictions. The protocol focuses on discovering structural classes of antibiotics but can be generally applied to other small molecules.

In this protocol, the tissue clearing process is carried out using a multiwell plate and insert, which allows the clearing steps to be conducted in parallel across multiple samples.

Generation and long-term culture of a human pluripotent stem cell-derived human cerebellar organoid model, which successfully replicates the cellular diversity of the fetal cerebellum along with some of its distinct cytoarchitectural features.

ChromEMT combines a fluorescent DNA binding dye that selectively enhances DNA and nucleosomes in electron microscopy with multi-tilt tomography, to enable the imaging and reconstruction of nuclear chromatin ultrastructure and 3D organization.

The facile synthesis of the 432 helicoid III nanoparticle morphology, displaying a Kuhn’s dissymmetry factor (g-factor) of 0.2, is detailed by using l-glutathione in an aqueous solution.

Kinetic rates of protein–protein interactions can be measured with high throughput via biolayer interferometry.